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비폴립에서 염증세포의 분포와 활성에 관한 면역조직화학적 및 전자현미경적 연구
Published Online: May 31, 2020
ABSTRACT
The pathogenesis of nasal polyps is not clearly defined yet, but it is believed that infection and IgE mediated allergy may play an important role in the pathogenesis of nasal polyp.
In the mucosa of nasal polyp, infUtration of various inflammatory cells (mainly eosinophils, neutrophils, lymphocytes, and mast cells) is found and they act as the effector cells and/or regulatory cells in the complex processes of the inflammatory response.
The studies on these inflammatory cells in nasal polyp may provide informations in the development of nasal polyp.
The author studied and compared the distribution pattern, number and activation status of the eosinophil and lymphocyte, and CD54 expression in the endothelium by immunohistochemical staining and fine structure of mast cell and eosinophil by electron microscope in 22 cases of nasal polyp with topical steroid treatment and 20 cases of untreated nasal polyp.
The purposes of this study are to know not only the role of the inflammatory cells in development of nasal polyp but also the effect of topical steroid treatment on inflammatory cells.
The results are as follows,
Majority of eosinophils in nasal polyp are found in the stromal layer and no significant difference in the density of eosinophils is observed between steroid treated group and untreated group, but the proportion of activated eosinophils (EG2𐀫 versus total eosinophil count) is significantly higher in untreated group than in the steroid-treated group.
The electron microscopic findings of activated eosinophil revealed loss of granular matrix, vacuolization, and loss of crystalloid core, and these findings are also more frequent in the untreated group than in the steroid-treated group.
Most of the T cells are CD3+, and the number of CD4+ (helper/induced) T cells present in nasal polyp is more than that of CD8+ (suppressor/cytotoxic) T cells.
The density of CD3+, CD4+, and CD8+ T lymphocytes is significantly decreased in the stromal layer in nasal polyp from steroid treated group, but no significant differences are found between two groups in the epithelial layen
The density difference of CD25+ T lymphocytes is not significant between two groups.
The density of CD54 expression of endothelium in the stromal layer of nasal polyp is significantly decreased in the steroid-treated group compared to untreated group.
The distribution density of mast cells shows no significant difference between two groups but the proportion of degranulated mast cells are significantly higher in untreated group.
These results suggested not only the effect of topical steroid in nasal polyp, which inhibits the activation of the eosinophil, and inhibit mast cell and CD54 expression of endothelium and reduced lymphocytes density, but also the presumptive role of activated eosinophil as well as mast cell in the development of nasal polyps.
Further study will be required for the evaluatuion of detailed function and role of various inflammatory cells related to the development of nasal polyp.