Journal of Clinical Otolaryngology Head and Neck Surgery

The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery

J Clin Otolaryngol Head Neck Surg 1994; 5(1):68-76

pISSN: 1225-0244, eISSN: 2713-833X

DOI: https://doi.org/10.35420/jcohns.1994.5.1.68

원저

아황산가스가 백서 기관상피에 미치는 영향에 관한 광학현미경 및 주사전자현미경적 연구

홍석찬¹, 추무진²

Light Microscopic and Scanning Electron Microscopic Study of Tracheal Epithelium by Exposure to Sulfur Dioxide Gas in Mice

Seok-Chan Hong¹, Moo-Jin Choo²

¹건국대학교 의과대학 이비인후과학교실

²충북대학교 의과대학 이비인후과학교실

¹Department of Otolaryngology, College of Medicine, Kon-Kuk University

²Department of Otolaryngology, College of Medicine, Chung-Buk University

© Copyright 1994 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: May 31, 2020

ABSTRACT

Sulfur dioxide, one of the important environmental pollutants, has been related to the increased morbidity rates or death due to respiratory damage. To investigate the effects of sulfur dioxide on the tracheal epithelium, and experiment was performed with 48 mice from the same colony. Experimental animals were divided into 3 groups, 30-minute exposure group, 60-minute exposure group, and 120-minute exposure group to 20 ppm sulfur dioxide gas.

Histopathological findings of mice exposed to sulfur dioxide were studied in each groups with light microscope immediately, 24 hours and 48 hours after sulfur dioxide exposure. No specific differences were noted among 3 group.

Scanning electron microscopic examination showed a tendency for ciliary compounding and ciliary lying-down in tracheal epithelium of mice exposed to sulfur dioxide. Ciliary compounding and lying-down were increased in severity as the exposure time was increased. However, ciliary compounding and lying-down disappeared 48 hours after exposure in all groups.

Keywords: Sulfur dioxide; Scanning electron microscope; Light microscope; Ciliary compounding and lying-down