Journal of Clinical Otolaryngology Head and Neck Surgery

The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery

J Clin Otolaryngol Head Neck Surg 2008; 19(1):63-71

pISSN: 1225-0244, eISSN: 2713-833X

DOI: https://doi.org/10.35420/jcohns.2008.19.1.63

원저

Prodigiosin이 두경부 종양세포주의 TRAIL매개 세포자멸사에 미치는 효과

김주연¹, 이강대¹^,^*, 이환호¹, 김우성¹, 이재훈¹, 박종욱²

Effect of Prodigiosin on TRAIL-Mediated Apoptosis of Head and Neck Cancer Cell

Joo Yeon Kim¹, Kang Dae Lee¹^,^*, Hwan Ho Lee¹, Woo Sung Kim¹, Jae Hoon Lee¹, Jong Wook Park²

¹고신대학교 의과대학 이비인후과학교실

²계명대학교 의과대학 면역학교실

¹Department of Otolaryngology Head and Neck Surgery, College of Medicine, Kosin University, Gospel Hospital, Busan, Korea

²Department of Immunology, Keimyung University, College of Medicine, Daegu, Korea

^*교신저자: 이강대, 602-702 부산광역시 서구 암남동 34 고신대학교 의과대학 이비인후과학교실 전화： (051) 990-6284·전송： (051) 245-8539 E-mail： kdlee@ns.kosinmed.or.kr

© Copyright 2008 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Apr 01, 2008; Accepted: Apr 29, 2008

Published Online: May 31, 2020

ABSTRACT

Background and Objectives: Prodigiosin (PG) is a red pigment produced by Seixatia marcescens with immu-nosuppressive and apoptotic activities. The purpose of this study was to evaluate the apoptotic effect and mechanism of PG on head and neck cancer, and show possibility of combination treatment with tumor necrosis factor related apoptosis inducing ligand (TRAIL) in TRAIL resistant cancer cells. Materials and Methods: Three head and neck cancer cell lines (AMC-HN4, AMC-HN6, AMC-HN9) were treated with PG and TRAIL. In order to know the mechanism of apoptosis, caspase 3 activity and apoptosis related protein expression were monitored by Western blotting. Results: TRAIL and PG induced apoptosis of AMC-HN9 cancer cells in dose- dependent manner. In AMC-HN4 cancer cells cotreated with PG and TRAIL, apoptosis was increased with up- regulation of PARP and DR5 expression and down-regulation of inactive form of caspase 3. Conclusions: PG effectively recovers TRAIL sensitivity in AMC-HN4, TRAIL and PG-resistant cancer cells, via DR5 up-regulation. PG alone or combination therapy with TRAIL could be a potent new strategy for treating head and neck cancer. (J Clinical Otolaryngol 2008;19:63–71)

Keywords: 두경부 종양; Prodigiosin; TRAIL; 세포자멸사

Keywords: Head and neck cancer; Prodigiosin; TRAIL; Apoptosis