Journal of Clinical Otolaryngology Head and Neck Surgery

The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery

J Clin Otolaryngol Head Neck Surg 2004; 15(1):103-108

pISSN: 1225-0244, eISSN: 2713-833X

DOI: https://doi.org/10.35420/jcohns.2004.15.1.103

원저

비인강암에서 중합효소반응을 이용한 유두종 바이러스의 검출

고의경¹, 이병주¹, 이현순¹, 조규섭¹, 노환중¹^,^*

Detection of Human Papillomavirus using Polymerase Chain Reaction(PCR) in Nasopharyngeal Carcinoma

Eui-Kyung Goh¹, Byung-Joo Lee¹, Hyun-Sun Lee¹, Kyu-Sup Cho¹, Hwan-Jung Roh¹^,^*

¹부산대학교 의과대학 이비인후과학교실

¹Department of Otolaryngology, College of Medicine, Pusan National University, Busan, Korea

^*교신저자: 노환중, 602-739 부산광역시 서구 아미동 1가 부산대학교 의과대학 이비인후과학교실 전화： (051) 240-7333·전송： (051) 246-8668 E-mail： rohhj@pusan.ac.kr

© Copyright 2004 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Apr 11, 2004; Accepted: May 13, 2004

Published Online: May 31, 2020

ABSTRACT

Background and Objectives: Nasopharyngeal carcinoma (NPC) is an unique epithelial malignancy which occurs at a high frequency in certain regions of Southeast Asia. Epstein-Barr virus (EBV) has been proposed to be closely associated with NPC. Human papillomavirus (HPV) is associated with a proportion of upper aero- digestive tract carcinomas. We hypothesized that HPV might also contribute to the pathogenesis of NPC. This study attempted to detect HPV DNA in nasopharyngeal carcinoma by polymerase chain reaction (PCR) . Materials and Methods: We reviewed thirty patients with nasopharyngeal carcinoma from 1995 to 2000 at the Department of Otolaryngology, Pusan National University Hospital. Thirty paraffin-embedded tissues were selected to PCR using type-specific primer pairs. Control group was selected twenty normal adenoid tissue. Results: HPV DNA was detected in 3/30 (10%) of NPC specimens, and in none of control specimens. WHO class I nasopharyngeal carcinomas (three cases) were all HPV type 6 positive. Conclusion: These results suggest that HPV type 6 may also be involved in the carcinogenesis of EBV-negative squamous cell nasopharyngeal carcinoma. (J Clinical Otolaryngol 2004;15:103–108)

Keywords: 비인강암; 중합효소연쇄반응; 유두종바이러스

Keywords: Nasopharyngeal carcinoma; PCR; HPV