Journal of Clinical Otolaryngology Head and Neck Surgery

The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery

J Clin Otolaryngol Head Neck Surg 1999; 10(2):202-210

pISSN: 1225-0244, eISSN: 2713-833X

DOI: https://doi.org/10.35420/jcohns.1999.10.2.202

원저

Mitogen 및 Mycoplasma 항원 자극시 사람 비강 섬유아세포에서 Nitric Oxide와 Interleukin-1의 생성에 미치는 효과

이한국¹^,^*, 김광혁², 유태현¹, 이강대¹, 이봉희¹, 이윤우¹, 장명웅²

Effects of Nitric Oxide and Interleukin-1 Produced by Human Nasal Fibroblast Stimulated by Mitogens and Mycoplasma Antigens

Han Kook Lee¹^,^*, Kwang Hyuk Kim², Tai Hyun Yu¹, Kang Dae Lee¹, Bong Hee Lee¹, Woon-Woo Lee¹, Myung Woong Chang²

¹고신대학교 의과대학 이비인후과학교실

²미생물학교실

¹Department of Otolaryngology-Head and Neck Surgery, Kosin University, Pusan, Korea

²Microbiology, College of Medicine, Kosin University, Pusan, Korea

^*교신저자: 이한국, 602-702 부산광역시 서구 암남동 34 고신대학교 의과대학 이비인후과학교실 전화： (051) 240-6277· 전송： (051) 245-8539 E-mail： hklee@ns.kosinmed.or.kr

© Copyright 1999 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Mar 10, 1999; Accepted: Apr 30, 1999

Published Online: May 31, 2020

ABSTRACT

Backround and Objectives: Excessive cytokine expression and nitric oxide (NO) induced by stimuli such as microbial products may be one aspect of the inflammatory response associated with bacteremia. We evaluated effects of mitogens and Mycoplasma antigens on the biological activity of human nasal fibroblast. Materials and Methods: We have undertaken a study of the kinetics of NO and interleukin-1 (IL-1) production in human nasal fibroblast culture exposed with lipopolysaccharide (LPS) of gram negative bacteria, Staphylococcus enterotoxin B (SEB), or Mycoplasma lysates. This study was designed to evaluate NO and IL-1 in the fibroblast culture supernatant by assays of nitrite ion concentration and mouse thymocyte cultivation. Results: 1) The NO production was increased by 0.01 μ/ml of LPS, all doses of SEB (0.001 -1.0 μ/ml), or all kinds of Mycoplasma lysates M. pneumoniae, M. fermentans, M. hominis), but decreased by 0.001, 0.1, and 1.0 μ/ml of LPS. 2) The IL-1 production was significantly increased by all doses of LPS (0.001 -1.0 μ/ml), SEB (0.001 -1.0 μ/ml), or Mycoplasma lysates. 3) In the culture supernatants of the fibroblast exposed to double stimuli, LPS plus SEB, LPS plus Mycoplasma lysates, the NO concentrations were higher than in those exposed to LPS alone. 4) The fibroblast responded differently to double exposure with stimuli in the production of IL-1. The IL-1 concentration increased or decreased according to doses and kinds of stimuli as compared with those exposed to LPS alone. Especially, the group exposured with LPS (0.01 μ/ml) plus Mycoplasma lysates showed highly significant increase of IL-1 as compared with the group exposed to LPS (0.01 μ/ml) alone. Conclusion: It is therefore believed that NO and IL-1 production in human nasal fibroblast incresed mostly by not only LPS, but also SEB. Moreover, its increase by Mycoplasma lysates was remarkable. Excessive NO and IL-1 production elicited by double exposure with stimuli in vivo may effect on the inflammatory reactions and cytokines production. (J Clinical Otolaryngol 1999;10:202–210)

Keywords: 섬유아세포 배양; 산화질소; Interleukin-1

Keywords: Human fibroblast culture; Lipopolysaccharide; Staphylococcus enterotoxin B; Mycoplasma lysates; Nitric oxide; Interleukin-1