원저

진주종 유래 섬유아세포에서의 Insulin-Like Growth Factor I과 Binding Protein의 역할

이창훈1, 조진생1,*, 박경유1, 김준우1, 이관원1, 이병권1, 이재수1
Chang Hoon Lee1, Chin Saeng Cho1,*, Kyung-You Park1, Joon Woo Kim1, Gwan Won Lee1, Byung Kwon Lee1, Jae Soo Lee1
Author Information & Copyright
1을지의과대학교 이비인후과학교실
1Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Eulji University, Deajeon, Korea
*교신저자: 조진생, 301-070 대전광역시 중구 목동 24 을지의과대학교 이비인후과학교실 전화: (042) 259-1324·전송: (042) 259-1321 E-mail: cscho@emc.eulji.ac.kr

© Copyright 2003 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Apr 06, 2003; Accepted: May 21, 2003

Published Online: May 31, 2020

ABSTRACT

Background and Objectives: Insulin like growth factor-I is an important growth regulator for epidermis and its activity is regulated by specific insulin like growth factor binding protein. Authors observed the expression of mRNA of Insulin-like Growth FactorI (IGF-I) and mRNA of Insulin-like Growth Factor Binding Protein (IGFBP) in fibroblast of patient with cholesteatoma to find out the function of fibroblast in the progress of cholesteatoma. Materials and Method: During the operation in middle ears, the cholesteatoma and normal skin tissue were extracted from 10 patients with Cholesteatoma. Some cytokines were added such as Interferon β, Interferon γ and TNF α to those extraced fibroblast. We analyzed the expression of IGF-I, IGFBP-3, and IGFBP-5 by semi-quantative RT-PCR. Results: After stimulation by inflammatory cytokine, the expression of IGF-I from cholesteatoma was significantly increased ,while the expression of IGFBP-5 was significantly reduced. Conclusion: These results indicate that cholesteatoma fibroblast could play an important role in the proliferation of cholesteatoma tissue. (J Clinical Otolaryngol 2003;14:113-117)

Keywords: 중이 진주종; 섬유아세포; 싸이토카인
Keywords: Cholesteatoma; Fibroblasts; Cytokines